Social Media Sidebar


Please sign up, comment on articles and bring your friends!

Current poll

PlanetTech is asking:

What do you think about our new web site?

Love it, indeed
Really good solution
Same as old one
The old one was better
This is a new option

Quote of the day

When seen through the lens of technology, few resources are truly scarce; they’re mainly inaccessible. 


Peter Diamandis

CRISPR enhancements: improving the ability to delete genes

Posted in Science on 9th Mar, 2016 04:20 PM by AlexMuller

Scientists have found a way to improve the efficiency of the gene editing technology, CRISPR/Cas9 (“CRISPR”). Lauded as a groundbreaking technology that allows scientists to modify genes* for many different applications, CRISPR/Cas9 has hit stormy waters over the ethics of editing human embryos.


Although the technology is faster and cheaper than past gene editing techniques, one of the problems cited is that the efficiency of deleting unwanted genes is low and that it gives inconsistent results, an unacceptable risk when using human embryos.

Haoquan Wu, PhD, from the Texas Tech University Health Sciences Center El Paso, has worked to improve CRISPR’s overall ability to target and eliminate genes.
In an open-access paper published in Genome Biology, senior author Wu and his team describe how they were able to significantly improve the efficiency of gene knockout (deletion) by creating structural changes to the CRISPR RNA guide molecule.**
“The extent of the improvement in knockout efficiency with these changes was striking,” Wu says. “This is going to help reduce concerns that knockout experiments might not work, and also significantly increase the efficiency of more challenging editing procedures like gene deletion.”
At this stage, the researchers are unsure why the changes to the single guide RNA enhanced CRISPR’s efficiency, although options include an enhanced ability to bind with Cas9 or improved stability of the RNA.
The TTUHSC El Paso team plans to continue studying this modified single guide RNA template to better understand why it enhances CRISPR’s functionality. They’ve also applied for a patent for their new method, which they hope will be adopted by other scientists using CRISPR.
CRISPR/Cas9 has two main components. A small “single guide” RNA molecule (Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR) directs the Cas9 enzyme to a specific sequence of DNA. The Cas9 then acts like molecular scissors to cut that specific piece of DNA. The DNA is then ready for editing.
Some technical aspects of CRSIPR-Cas9 are inefficient, including total gene knockout. To improve the efficiency, the researchers optimized the structure of the small-guide RNA by tweaking the sequence and making it slightly longer.
The optimized small-guide RNA was far more efficient at producing knockouts, 18 of 24 experiments produced greater than 50 per cent knockout efficiency compared with only four for the original small-guide RNA structure.

Tags: geneticsmedicinebiotechnologyCRISPRDNA

Read original article » Back to category


Author: Guest
Posted: 2016-03-10
CRISPR is definitely a breakthrough but needs further improvements to be applicable more widely and to a range of genes and a range of mutations - improving any aspect is a step forward Reply


Recent headlines

  • Posted in Medicine on 2018-03-17 19:49:27
    A simple artificial heart could permanently replace more
    Posted in Software on 2018-03-16 11:03:28
    60.3% of harmful Android apps in 2017 detected more
    Posted in Hardware on 2018-03-16 11:00:28
    Big harpoon is 'solution to space junk' more
    Posted in Science on 2018-03-16 10:58:40
    Russia will beat NASA to mars and find water on the moon, more
    Posted in Business on 2018-03-14 07:02:30
    X Prize announces $10 million competition to more
Posted in Business on 2013-10-10 01:33
China is working towards a manned lunar mission in more
Posted in Business on 2013-10-20 07:17
Spacex says China is their main competitor for more
Posted in Software on 2013-10-20 06:43
Pirate Bay Browser Clocks 1,000,000 more
Posted in Medicine on 2013-10-10 02:10
Google reportedly investing hundreds of millions into more
Posted in Medicine on 2013-10-14 03:13
Endothelial Cells Can Repair and Regenerate Organs, more
Posted in Science on 01.01.2010
Spacex says China is their main competitor for more
Posted in Science on 01.01.2010
Staring at Your Phone Could Be Making You Short more
Posted in Science on 01.01.2010
Oculus Rift virtual reality headset coming to mobile, more
Posted in Science on 01.01.2010
China is working towards a manned lunar mission in more
Posted in Science on 01.01.2010
Delivering drugs via nanoparticles to target more

Recent Blog Posts

  • Posted by AlexMuller
    Martian landscapes formed from sand 'levitating' on a more
    Posted by AlexMuller
    Scientists detect comets outside our solar more
    Posted by AlexMuller
    Advanced artificial limbs mapped in the more
    Posted by AlexMuller
    Bacteria have a sense of more
    Posted by AlexMuller
    Recurrent brain cancer: New hope with phase 1 clinical more

Login to your Account

Login to your PlanetTech Account here

Remember me

Create a New Account

You just need username and password

The following errors occured:
Verify password:
Remember me