Social Media Sidebar

Announcement

Please sign up, comment on articles and bring your friends!

Current poll

PlanetTech is asking:

What do you think about our new web site?

Love it, indeed
Really good solution
Same as old one
The old one was better
This is a new option

Quote of the day

When seen through the lens of technology, few resources are truly scarce; they’re mainly inaccessible. 

 

Peter Diamandis

Retinal nerve cells grown in the lab which could lead to cell transplants for blind people

RATE THIS! +45
Posted in Science on 4th Dec, 2015 05:28 PM by AlexMuller

Researchers have developed a method to turn human stem cells into retinal ganglion cells, nerve cells located within the retina that transmit visual signals from the eye to the brain. Death and dysfunction of these cells cause vision loss in glaucoma and multiple sclerosis.

 
"Our work could lead not only to a better understanding of the biology of the optic nerve, but also to a cell-based human model that could be used to discover drugs that stop or treat blinding conditions," says study leader Donald Zack, M.D., Ph.D., the Guerrieri Family Professor of Ophthalmology at the Johns Hopkins University School of Medicine. "And, eventually it could lead to the development of cell transplant therapies that restore vision in patients with glaucoma and MS."
 
The laboratory process, described in the journal Scientific Reports, entails genetically modifying a line of human embryonic stem cells to become fluorescent upon their differentiation to retinal ganglion cells, and then using that cell line for development of new differentiation methods and characterization of the resulting cells.
 
Using a genome editing laboratory tool called CRISPR-Cas9, investigators inserted a fluorescent protein gene into the stem cells' DNA. Researchers found that adding a naturally occurring plant chemical called forskolin on the first day of the process helped improve the cells' efficiency of becoming retinal ganglion cells.
 
"By the 30th day of culture, there were obvious clumps of fluorescent cells visible under the microscope," says lead author Valentin Sluch, Ph.D., a former Johns Hopkins biochemistry, cellular and molecular biology student and now a postdoctoral scholar working at Novartis, a pharmaceutical company. Sluch completed this research at Johns Hopkins before transitioning to Novartis.
 
"I was very excited when it first worked," Sluch says. "I just jumped up from the microscope and ran [to get a colleague]. It seems we can now isolate the cells and study them in a pure culture, which is something that wasn't possible before."
 
"We really see this as just the beginning," adds Zack. In follow-up studies using CRISPR, his lab is looking to find other genes that are important for ganglion cell survival and function. "We hope that these cells can eventually lead to new treatments for glaucoma and other forms of optic nerve disease."

Tags: eyevisionstem cellsblindnessresearchmedicineCRISPR

Read original article » Back to category

Comments

Author: Guest
Posted: 2015-12-05
+1
Regenerative medicine is clearly benefiting from modern methods in gene editing and hopefully this is going to be safe so it can find its to the clinic
1 Replies
Author: Guest
Posted: 2015-12-05
+0
Yes, we can expect many applications and this example of using CRISPR to help with stem cell application in cure of blindness is very hopeful Reply
Reply


 

Recent headlines

  • Posted in Software on 2017-12-17 22:27:47
    Ethereum, Litecoin and Ripple added to Bloomberg.....read more
    Posted in Science on 2017-12-17 22:22:12
    Blue Origin flies booster and crew capsule..read more
    Posted in Medicine on 2017-12-17 22:19:29
    Anti-aging drugs only a few years away..read more
    Posted in Online on 2017-12-15 13:43:41
    The FCC votes to repeal the 2015 net neutrality rules..read more
    Posted in Science on 2017-12-15 13:34:37
    How Clinical Trials Are Making the Case for MDMA in.....read more
Posted in Business on 2013-10-10 01:33
China is working towards a manned lunar mission in about.....read more
Posted in Business on 2013-10-20 07:17
Spacex says China is their main competitor for commercial.....read more
Posted in Software on 2013-10-20 06:43
Pirate Bay Browser Clocks 1,000,000 Downloads..read more
Posted in Medicine on 2013-10-10 02:10
Google reportedly investing hundreds of millions into new.....read more
Posted in Medicine on 2013-10-14 03:13
Endothelial Cells Can Repair and Regenerate Organs,.....read more
Posted in Science on 01.01.2010
Spacex says China is their main competitor for commercial.....read more
Posted in Science on 01.01.2010
Staring at Your Phone Could Be Making You Short Sighted..read more
Posted in Science on 01.01.2010
Oculus Rift virtual reality headset coming to mobile, but.....read more
Posted in Science on 01.01.2010
China is working towards a manned lunar mission in about.....read more
Posted in Science on 01.01.2010
Delivering drugs via nanoparticles to target mitochondria..read more

Recent Blog Posts

  • Posted by AlexMuller
    Martian landscapes formed from sand 'levitating' on a little.....read more
    Posted by AlexMuller
    Scientists detect comets outside our solar system..read more
    Posted by AlexMuller
    Advanced artificial limbs mapped in the brain..read more
    Posted by AlexMuller
    Bacteria have a sense of touch..read more
    Posted by AlexMuller
    Recurrent brain cancer: New hope with phase 1 clinical trial.....read more

Login to your Account

Login to your PlanetTech Account here

Username:
Password:
Remember me
or

Create a New Account

You just need username and password

The following errors occured:
Username:
Email:
Password:
Verify password:
Remember me