Social Media Sidebar

Announcement

Please sign up, comment on articles and bring your friends!

Current poll

PlanetTech is asking:

What do you think about our new web site?

Love it, indeed
Really good solution
Same as old one
The old one was better
This is a new option

Quote of the day

'By far the greatest danger of Artificial Intelligence is that people conclude too early that they understand it'

 

Eliezer Yudkowsky

Super-resolution 3D microscopy images cells in unprecedented detail

RATE THIS! +48
Posted in Science on 17th Aug, 2016 03:42 PM by AlexMuller

A new ultra-high resolution "nanoscope" is capable of taking 3D images of an entire cell and its constituents in unprecedented detail, an advance that could reveal biological phenomena never before seen and bring new medical insights. The work was carried out by various universities and institutions.

 
The technology incorporates several innovations in fluorescence microscopy and super-resolution microscopy and harnesses the same kind of "adaptive optics" technology used in astronomy, deformable mirrors that change shape to compensate for light distortion.
 
In astronomy the deformable mirrors are used to compensate for atmospheric distortion to yield clear images of celestial objects. Deformable mirrors also can be used to counteract the distortion caused when light passes through biological tissue.
 
The technology was demonstrated by imaging cellular components, including synaptonemal complexes, which link chromosomes together; mitochondria and the endoplasmic reticulum, which are critical for cellular functions; the cilia, which protrude from cells like tiny antennas; and bacteriophages, which are viruses about 50 nanometers in diameter that infect bacteria.
 
The new system advances the original super-resolution fluorescence microscopy by incorporating the deformable mirrors into a microscope using two objectives, one above and one below the sample, and introducing a set of new algorithms to pinpoint molecular positions of proteins deep inside cells.
 
The system allows researchers to resolve details far smaller than the wavelength of light, representing a powerful and versatile new laboratory tool, Huang said.
 
"The wave nature of light restricts the resolution of conventional light microscopy to about 200 nanometers, making details of subcellular structures and protein assemblies unresolvable," Huang said.
 
The new system, however, allows imaging of cellular constituents in 3-D at 10- to 20-nanometer resolution throughout entire mammalian cells, powerful enough to reconstruct the fine features of viruses. Until now, resolving such fine details was only possible using electron microscopy, which requires samples to be treated, killing the cells.
 
"One goal is to further push the envelope in the direction of live-cell and tissue imaging, two major roadblocks of modern super-resolution techniques," Huang said, "and therefore allow visualization of cellular functions live in their physiological conditions at the nanoscale."
 
Molecules inside cells and in structures called organelles can be tagged with either "photo-switchable" fluorescent proteins or organic dyes that are able to glow when exposed to a small amount of ultraviolet light.
 
"These special fluorescent tags (fluorophores) have two states, on and off," Huang said. "And you can control the on and off states by shining light on these molecules. The concept of single molecule switching nanoscopy is to stochastically switch molecules on and off at different time frames, pinpoint the exact locations of single molecules and reconstruct the cellular constituents at super resolution."
 
The super-resolved images are reconstructed from the positions of thousands to millions of single molecules.
 
"We are interested in using our developments to study the cytokinetic apparatus, a core machinery during cell division," Huang Said.

Tags: microscopecellbiologyimagingmedicinehardware

Read original article » Back to category

Comments

Author: Guest
Posted: 2016-08-18
+1
This is very significant advance and the super resolution 3D microscope will allow not only insights into structural detail but also into monitoring changes in live cells
1 Replies
Author: Guest
Posted: 2016-08-18
+0
Indeed, the ability to access detail in non-fixed, live cells will provide information in real time and be more relevant functionally Reply
Reply


 

Recent headlines

  • Posted in Business on 2017-03-24 19:18:37
    This company replaced 90% of its workforce with machines......read more
    Posted in Hardware on 2017-03-24 19:07:39
    China working on reusing rocket stages and will try to.....read more
    Posted in Medicine on 2017-03-24 18:59:31
    Discovery enables 'mass produced blood'..read more
    Posted in Hardware on 2017-03-23 21:29:30
    A brain-computer interface for controlling VR with your mind..read more
    Posted in Business on 2017-03-23 21:17:44
    One Powerful Way the U.S. Could Boost Solar Adoption..read more
Posted in Business on 2013-10-10 01:33
China is working towards a manned lunar mission in about.....read more
Posted in Business on 2013-10-20 07:17
Spacex says China is their main competitor for commercial.....read more
Posted in Software on 2013-10-20 06:43
Pirate Bay Browser Clocks 1,000,000 Downloads..read more
Posted in Medicine on 2013-10-10 02:10
Google reportedly investing hundreds of millions into new.....read more
Posted in Medicine on 2013-10-14 03:13
Endothelial Cells Can Repair and Regenerate Organs,.....read more
Posted in Science on 01.01.2010
Spacex says China is their main competitor for commercial.....read more
Posted in Science on 01.01.2010
Staring at Your Phone Could Be Making You Short Sighted..read more
Posted in Science on 01.01.2010
Oculus Rift virtual reality headset coming to mobile, but.....read more
Posted in Science on 01.01.2010
China is working towards a manned lunar mission in about.....read more
Posted in Science on 01.01.2010
Delivering drugs via nanoparticles to target mitochondria..read more

Recent Blog Posts

  • Posted by AlexMuller
    Intensive medical treatment can reverse type 2 diabetes..read more
    Posted by AlexMuller
    Daily consumption of tea protects the elderly from cognitive.....read more
    Posted by AlexMuller
    New analysis method of metabolites accurately predicts whether.....read more
    Posted by AlexMuller
    Unproven stem cell 'therapy' blinds three patients at Florida.....read more
    Posted by AlexMuller
    Indigenous South American group has healthiest arteries of all.....read more

Login to your Account

Login to your PlanetTech Account here

Username:
Password:
Remember me
or

Create a New Account

You just need username and password

The following errors occured:
Username:
Email:
Password:
Verify password:
Remember me