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The Nobel Prize in Chemistry 2014: beyond the diffraction limit in microscopy

RATE THIS! +38
Posted in Science on 11th Oct, 2014 11:56 PM by AlexMuller

For a long time optical, microscopy was held back by a presumed limitation: that it would never obtain a better resolution than half the wavelength of light, the academy notes in a statement.“Helped by fluorescent molecules, the Nobel Laureates in Chemistry 2014 ingeniously circumvented this limitation.”

 

“Their ground-breaking work has brought optical microscopy into the ‘nanodimension. In what has become known as nanoscopy, scientists visualize the pathways of individual molecules inside living cells. They can see how molecules create synapses between nerve cells in the brain; they can track proteins involved in Parkinson’s, Alzheimer’s and Huntington’s diseases as they aggregate; they can follow individual proteins in fertilized eggs as these divide into embryos.”

 
In 1873, the microscopist Ernst Abbe stipulated a physical limit for the maximum resolution of traditional optical microscopy: 0.2 micrometers, or 200 nanometers (the shortest wavelength for visible light, the extreme limit of violet). The three Nobel laureates bypassed this limit, extending our view into the nanoworld.

Tags: lightmicroscopemicroscopy hardware

Read original article » Back to category

Comments

Author: Guest
Posted: 2014-10-12
+1
Well deserved! This is one of the methods that will change microscopy and add important functional aspect to observation well beyond just fixed images Reply
Author: Guest
Posted: 2016-12-26
+0
How neat! Is it really this silmep? You make it look easy. Reply


 

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